Table 4.
PCR step | SNV segment | Primer name | Sequence |
RT | S | SNV-S5'T | TAGTAGTAGACTTCKTRAAGAGCTACT |
1° reaction | S | SNV-S41s | GGAATGAGCACCCTCAAAGAAGTGCAAGACAAC |
1° reaction | S | PSE-S1064r | ATRGTRTTYCTCATATCCTG |
2° reaction | S | SNV-S143s | TGGACCCMGATGAYGTTAACAA |
2° reaction | S | SNV-S1000r | GACAYCGATCWGGNGCACATGCAAARACCC |
RT | M | SNV-M2730s | CTTTTAGAAAGAWMTGTGSRTTTGC |
1° reaction | M | SNV-M2730s | CTTTTAGAAAGAWMTGTGSRTTTGC |
1° reaction | M | SNV-M3023r | CCTACTCCTGAACCCCAGGCCCCG |
2° reaction | M | SNV-M2764s | CCAACATGTGAGTATCAAGGCAACACAGTGCTGG |
2° reaction | M | SNV-M2966r | GGKKTWTCACTTAGRTCYTGRAAGG |