Table 4.
Localisation and evaluation of differences between 43RCA framework regions, and those coded by 43RCA most similar human germline genes
| Total | Identical | Very similar (+++) |
Similar (+-+), (++-), (+-+) |
Dissimilar (--+), (-+-), (+- -) |
Very dissimilar (- - -) |
|
| VH | ||||||
| FR1-IMGT | 25 | 23 | 0 | 2 | 0 | 0 |
| FR2-IMGT | 17 | 14 | 0 | 1 | 0 | 2 |
| FR3-IMGT | 38 | 34 | 0 | 1 | 3 | 0 |
| FR4-IMGT | 11 | 9 | 0 | 1 | 0 | 1 |
| FR-IMGT | 91 | 80 | 0 | 5 | 3 | 3 |
| V-KAPPA | ||||||
| FR1-IMGT | 26 | 24 | 1 | 1 | 0 | 0 |
| FR2-IMGT | 17 | 15 | 0 | 0 | 2 | 0 |
| FR3-IMGT | 36 | 33 | 0 | 1 | 1 | 1 |
| FR4-IMGT | 10 | 10 | 0 | 0 | 0 | 0 |
| FR-IMGT | 89 | 82 | 1 | 2 | 3 | 1 |
| All FR-IMGT VH and V-KAPPA | 180 | 162 | 1 | 7 | 6 | 4 |
| (%) | 90% | 0.56% | 3.89% | 3.33% | 2.22% |
Differences between 43RCA VH and VL framework amino acids, and those coded by most similar human genes, are evaluated on a 5-level scale (from identical to very dissimilar, see text) and located in each framework region.