. Author manuscript; available in PMC: 2010 Aug 14.

Published in final edited form as: J Mol Biol. 2009 Jun 6;391(2):375–389. doi: 10.1016/j.jmb.2009.06.011

Table 2.

Thermodynamic parameters for ZnF-Ran binding in Isothermal Titration Calorimetry experiments.

Titrand	[Titrand] (μM)	Titrant	[Titrant] (mM)	T (K)	K_{d 1} (μM)	N₁	K_{d 2} (μM)	N₂	ΔH₁ / ΔH₂ (kJ/mol)	TΔS₁ / TΔS₂ (kJ/mol)
`Nup153ZnF1234`	`12`	`RanGDP`	`0.31`	`293`	`1`	`2`	`8`	`2`	`−40.8 / 3.9`	`−7.38 / 32.7`
”	`12`	`RanQ69L`	`0.40`	`293`	`20`	`2`	`-`	`-`	`−39.3/ -`	`−12.9 / -`
`Nup153-ZnF12`	`15`	`RanGDP`	`0.29`	`293`	`4`	`1`	`57`	`1`	`−41.0 / 20.4`	`−10.3 / 44.2`
`Nup153-ZnF34`	`23`	`RanGDP`	`0.35`	`293`	`3`	`1`	`59`	`1`	`−17.2 / −2.2`	`14.0 / 21.6`
`Nup153-ZnF1`	`13`	`RanGDP`	`0.30`	`293`	`6`	`1`	`-`	`-`	`−7.8`	`21.4`
`Nup153-ZnF1Q8D`	`20`	`RanGDP`	`0.36`	`293`	`8`	`1`	`-`	`-`	`−11.2`	`17.6`
`Nup153-ZnF2`	`32`	`RanGDP`	`1.25`	`278`	`49`	`1`	`-`	`-`	`16.8`	`39.8`
`Nup153-ZnF2D8Q`	`18`	`RanGDP`	`0.34`	`293`	`4`	`1`	`-`	`-`	`−5.5`	`24.9`
`Nup153-ZnF3`	`29`	`RanGDP`	`0.30`	`293`	`7`	`1`	`-`	`-`	`−3.1`	`26.0`
`Nup153-ZnF3E8D`	`17`	`RanGDP`	`0.34`	`293`	`-`	`-`	`-`	`-`	`-`	`-`
`Nup153-ZnF4`	`50`	`RanGDP`	`0.95`	`278`	`47`	`-`	`-`	`-`	`12.6`	`35.7`
`Nup153-ZnF4D8Q`	`19`	`RanGDP`	`0.36`	`293`	`3`	`1`	`-`	`-`	`−6.6`	`24.3`
`Nup358-ZnF12`	`25`	`RanGDP`	`1.25`	`278`	`15`	`2`	`-`	`-`	`−3.4`	`22.5`