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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Biotechnol Bioeng. 2009 Sep 1;104(1):206–215. doi: 10.1002/bit.22378

Figure 5.

Figure 5

In vivo targeted transduction of Jurkat.hcKIT cells xenografted by intra-bone marrow injection with engineered lentiviral vectors. (A) Schematic diagram of the procedure used to target cell in the bone marrow. Jurkat.hcKIT cells (1 × 106) were injected into the right femur of the mice. One week later lentivector (1 × 106 TU) expressing both firefly luciferase and eGFP either targeted (FUGL/hSCF+FM3) or control vector (FUGL/CD20+SGM) was injected into the right femur of the mouse. (B) One week after the injection of lentivector, mice were injected with the substrate D-luciferin and analyzed using bioluminescence imaging. The experiment was repeated twice and one representative result is shown with luminescence quantification. A mouse receiving Jurkat.hcKIT with no lentivector was used as a negative control. (C) Mice were culled and the cells from the right femur were collected and cultured. FACS analysis was used to determine CD117 and eGFP expression.