Cell-free BAL samples were analyzed by ELISA (A), (C), and (D) or by cytotoxicity bioassay (B) for the concentrations (pg/ml) of TNFα(A), (B), IL-1β (C), and IL-10 (D). Levels of TNFα, IL-1β, and IL-10 in uninjured control rats were at or below the lower limit of detection of the assays used. Data are mean ± SEM for n = 6–12 rats. The same statistical analyses as in Fig. 1 were performed. *P < 0.002 compared with uninjured controls; **P < 0.003 compared with LC alone; ***P < 0.004 compared with CASP alone.