Table 1.
Catalytic activities of EUK-400 series compounds compared with those of EUK-189 and EUK-207
| Compound | SOD IC50 (μM) | CAT (μM H2O2 min−1) |
POD (μM TMB min−1) |
|---|---|---|---|
| EUK-189 | 0.37 | 3.15 ± 0.01 | 3.61 ± 0.05 |
| EUK-207 | 0.48 | 3.21 ± 0.04 | 4.43 ± 0.003 |
| EUK-418 | 1.73 | 0.47 ± 0.10 | 0.63 ± 0.08 |
| EUK-423 | 6.36 | 0.45 ± 0.18 | 0.61 ± 0.05 |
| EUK-425 | 12.5 | 0.57 ± 0.15 | 0.58 ± 0.03 |
| EUK-450 | 6.39 | 0.58 ± 0.08 | 0.66 ± 0.10 |
| EUK-451 | 3.36 | 0.64 ± 0.25 | 0.59 ± 0.023 |
| EUK-452 | 11.6 | 0.54 ± 0.06 | 0.63 ± 0.05 |
| EUK-453 | 5.95 | 0.50 ± 0.11 | 0.56 ± 0.03 |
Assays were performed as described in “Materials and methods.” EUK-400 series compounds were tested from dimethyl sulfoxide stock solutions for superoxide dismutase (SOD) and catalase (CAT) activities, and from H2O for peroxidase (POD) activity. EUK-189 and EUK-207 were diluted from a H2O stock solution. Standard deviations of triplicate samples are given for CAT and POD. Under these conditions, Cu/Zn SOD enzyme exhibited an IC50 value of 0.0011 μM
TMB tetramethyl benzidine