Figure 1.
(A) Sequences of HCV p7 protein from the H77 and JFH-1 strains. The gray box indicates H-17. Cylinders indicate predicted helical regions. (B) Typical channel recording traces. (C) Time course of inhibition of the wild-type H77 p7 ion channel protein by 50 μM (gray squares) and 500 μM (black diamonds) Cu2+. The symbols in this and the following figures show the recorded currents (mean ± SE for n = 10), and the lines show the currents fit with an optimal exponential decay constant using MATLAB (The MathWorks, Natick, MA). (D) Time course of inhibition by 1mM Mg2+. At t = 1400 s, 500 μM of NN-DNJ was added to confirm the presence of p7 ion channels.