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E2 (17β-estradiol) regulation of survival pathways in wild-type MCF-7 cells and long-term estrogen-deprived MCF-7:5C and MCF-7:2A breast cancer cells. Cells were treated with 1 nM E2 for 72 hours, and protein lysates were analyzed by Western blot for p-Akt, Akt, and nuclear factor-kappa-B (NF-κB). β-actin was used as a loading control.
