Figure 5.

Contribution of RNA2 subdomains to 2a accumulation defect. (A) Alignment of RNA1 and RNA2 5′ NCR sequences shows subdomains A, B, and C. Identical residues are highlighted in black and similar residues (purines or pyrimidines) are on a shaded background. Sequences predicted to be paired in subdomain A are overlined or underlined. (B) Schematic diagram of deletions in 2a mRNA 5′ NCRs and Northern and Western blot analyses are shown as in Fig. 4A of wt and ded1i yeast containing these mRNAs. (C) Relative accumulation of 2a protein expression from mRNAs diagramed in B is shown. Data are averages of three or more experiments.