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. 2009 Aug 6;4(8):e6538. doi: 10.1371/journal.pone.0006538

Figure 6. Expression and turnover of Sheep Sho alleles in transfected N2a cells.

Figure 6

(A) GFP fluorescent signals in fields of transfected cells to demonstrate similar transfection efficiencies. Individual values were V71 (10.1%), A71 (5.1%), “+9” (8.7%), “−12” (7.1%). (B) Western blot analysis of N2a transfected cells chased for different time periods in the presence of cycloheximide. Proteins samples are de-glycosylated with PNGase F. Positions of full-length and C1 fragments of sheep Sho are indicated, detected with a C-terminal anti-Sho antibody.