TABLE 5. iTRAQ Identification of Proteins Specifically Down-regulated by Egln1 Knockdown in VHL(−) Renal Carcinoma Cells.
Fold change in the protein level VHL(−)/Egln1 kd cells vs. VHL(−) cells. 2-fold difference: below 0.528; 0.75 fold-difference: below 0.603; 0.6-fold difference: below 0.660; 0.5-fold difference: below 0.704.
| Protein | Function | Accession No. | Protein MW | Protein PI | Pep. Count | Total Ion Score | Fold Change |
|---|---|---|---|---|---|---|---|
| ANXA1 | Annexin 1 | gi|4502101 | 43445.4 | 6.57 | 10 | 929 | 0.6477 |
| GDI2 | Human rab GDI | gi|285975 | 56107.7 | 5.94 | 4 | 436 | 0.6941 |
| HADHA | Mitochondrial trifunctional protein, alpha subunit precursor | gi|20127408 | 93322.2 | 9.16 | 3 | 314 | 0.6951 |
| P4HB | Prolyl 4-hydroxylase, beta subunit precursor | gi|20070125 | 63997.6 | 4.76 | 4 | 278 | 0.6774 |
| CNN3 | Calponin 3 | gi|4502923 | 39993.3 | 5.69 | 3 | 248 | 0.5275 |
| SET | SET translocation isoform 1 | gi|170763500 | 36927.1 | 4.23 | 2 | 244 | 0.5985 |
| AKR1B1 | Aldo-keto reductase, member B1 | gi|4502049 | 39577.1 | 6.51 | 3 | 236 | 0.6686 |
| CCT3 | T-complex polypeptide 1 | gi|36796 | 66119.8 | 6.03 | 2 | 159 | 0.678 |
| LARP3 | Chain A, Crystal Structure Of N-Terminal Domains Of Human La Protein | gi|187609149 | 26227.3 | 6.28 | 2 | 156 | 0.5833 |
| ASPH | Aspartyl (asparaginyl) beta- hydroxylase | gi|1911652 | 93619.0 | 4.92 | 3 | 152 | 0.5246 |
| PLOD | Lysyl hydroxylase | gi|190074 | 88859.2 | 6.47 | 2 | 150 | 0.6269 |
| COPE | Epsilon subunit of coatomer protein complex isoform a | gi|31542319 | 36621.9 | 4.97 | 1 | 137 | 0.6998 |
| NASP | Histone-binding protein | gi|184433 | 94361.6 | 4.27 | 2 | 135 | 0.5227 |
| PARP1 | Poly(ADP-ribose) polymerase | gi|190167 | 131312.4 | 9.02 | 1 | 129 | 0.6903 |
| VAT1 | VAT1 protein | gi|32425722 | 44457.4 | 6.17 | 1 | 128 | 0.5161 |
| CPSF6 | Cleavage and polyadenylation specific factor 6, 68 kD subunit | gi|162329583 | 61623.1 | 6.66 | 1 | 115 | 0.5634 |
| SLC7A5 | Solute carrier family 7, member 5 | gi|24981008 | 58128.6 | 8.17 | 1 | 104 | 0.6042 |
| NHP2L1 | PREDICTED similar to Nhp2 non-histone chromosome protein 2-like 1 | gi|109094377 | 8759.8 | 7.74 | 1 | 101 | 0.6932 |
| SMEK1 | SMEK homolog 1 | gi|74736507 | 104098.2 | 4.83 | 1 | 99 | 0.6894 |
| PRDX6 | Peroxiredoxin 6 | gi|54303999 | 10577.5 | 5.51 | 1 | 92 | 0.4612 |
| GLS | Glutaminase isoform C | gi|6002671 | 63556.8 | 6.05 | 1 | 90 | 0.5805 |
| ARL6IP5 | ADP-ribosylation-like factor 6 interacting protein 5 variant | gi|62898864 | 23127.5 | 9.88 | 1 | 90 | 0.589 |
| HPIBP3 | HP1-BP74 | gi|56676330 | 72697.4 | 9.69 | 1 | 86 | 0.482 |
| HIPIR | KIAA0655 protein | gi|3327124 | 130482.6 | 6.41 | 1 | 81 | 0.6648 |
| CALR* | Calreticulin-calcium binding protein | gi|913156 | 11943.6 | 4.12 | 1 | 78 | 0.6051 |
| RNASET2 | Ribonuclease T2, isoform CRA e | gi|119567903 | 21813.4 | 8.14 | 1 | 76 | 0.4669 |