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. 2009 Feb 4;96(3):1210–1217. doi: 10.1016/j.bpj.2008.10.031

Figure 3.

Figure 3

Effect of TBT-Cl on the rotation of V1 at a low ATP concentration. (A) Time courses of rotation in the absence (red line and dots) and presence (blue line and dots) of 10 μM TBT-Cl. (Inset) Traces of the centroid of the bead images. (B) Histogram of pausing time at 10 μM ATP in the presence of 10 μM TBT-Cl. It was fitted with sum of two exponential, y = C1 × exp(−t/τ1) + C2 × exp(−t/τ2) (τ1 < τ2). Estimated time constants, shown on the figure and in Table 1. (τ1 value was calculated to be 0.11 ± 0.01, which was below the bin width.) Total counts of events were 713 (8 molecules). (Inset) Histogram of pausing time at 10 μM ATP with no TBT-Cl. The line is fit with y = C × exp(−t/τATP). Total counts of events were 795 (9 molecules). Blue lines show the result of a global fit for these two histograms with time constants τATP = 0.20 ± 0.01 and τoff = 2.16 ± 1.03. (C) Frequency of pausing time at 10 μM ATP with no TBT-Cl (black bar) and 10 μM TBT-Cl (gray bar).