Table 1.
Kinetic parameters of the effect of TBT-Cl on V1
| ATP (μM) | TBT-Cl (μM) | τoff (s) | τon (s) | kon (105 M−1s−1) | Kd (μM) | τATP (s) |
|---|---|---|---|---|---|---|
| 4000 | 0 | — | — | — | — | — |
| 10 | 0.43 ± 0.04 | 0.71 ± 0.02 | 1.4 ± 0.0 | 16.6 | — | |
| 50 | 0.51 ± 0.03 | 0.20 ± 0.01 | 1.0 ± 0.0 | 19.6 | — | |
| 10 | 0 | — | — | — | — | 0.23 ± 0.01 |
| 10 | 0.94 ± 0.8 | — | — | — | (0.11 ± 0.01) |
Each value was obtained from single-molecule measurements of V1 under several conditions (mean ± SE). τon and τoff are the time constants of TBT-Cl-bind and-release to/from V1. The values were calculated from the histogram of moving times (τon) and pausing times (τoff) (Figs. 2 and 3). Kd values were calculated from koff /kon. Dash means undetermined parameters.