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. 2009 Feb 4;96(3):1016–1025. doi: 10.1016/j.bpj.2008.10.032

Figure 2.

Figure 2

IRRA spectra of GST-PI3Kγ on Tris buffer at 9 mN/m acquired with p-polarized light at 40° and 62° (solid lines). Inserted are the corresponding simulated spectra (dotted lines) for (A) an α-helical structure lying flat at the interface, (B) a β-sheet lying flat at the air-buffer interface, and (C) a random coil structure. The spectra were calculated for an α-helix with a length of 1.5 nm and a width of 0.5 nm, and a β-sheet with a film thickness of 1 nm and the extinction coefficients of a model antiparallel β-sheet (47). The marked positions of the main bands are taken from Goormaghtigh et al.(48).