IRRA spectra of the Tris buffer surface with p-polarized light at 40° angle of incidence covered with (A) DSPC (top) and DSPA (bottom), with (solid line) and without (dashed line) GST-PI3Kγ at 5 mN/m; (B) DMPC (top) and DMPA (bottom), with GST-PI3Kγ at 5 mN/m (solid line), at 10 mN/m (dotted line), and without protein (dashed line). (C) SAPA with GST-PI3Kγ at 5 mN/m (solid line), at 30 mN/m (dotted line), and without GST-PI3Kγ (dashed line).