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Influence of the fatty-acid pattern on GST-PI3Kγ affinity to PtdIns(4,5)P2 monolayers. IRRA spectra of DP(16:0)- (dotted line), DO(18:1)- (dashed line), and stearoyl-arachidonoyl(18:0, 20:4)-PtdIns(4,5)P2 (solid line) with adsorbed GST-PI3Kγ on Tris buffer at 20 mN/m and acquired with p-polarized light at 40° angle of incidence. The kinase was injected at 5 mN/m. After protein adsorption, the film was compressed to 20 mN/m.
