Table 2.

Apparent destabilization energies (ΔΔG) at the mitochondrial surface at two different ionic strengths in kcal/mol

Mutation in barnase*	I = 0.17 M†§	I = 0.05 M‡§
Ile4 → Ala	0.6  ± 0.1	0.7  ± 0.2
His18 → Gln	0.7  ± 0.1	0.7  ± 0.2
Asn23 → Ala	1.4  ± 0.1	1.0  ± 0.2
Ile25 → Val	0.7  ± 0.1	0.8  ± 0.2
Thr26 → Ala	1.0  ± 0.2	1.2  ± 0.2
Val36 → Ala	0.6  ± 0.1	0.8  ± 0.1
Ile51 → Val	1.1  ± 0.2	1.1  ± 0.2
Asp54 → Ala	1.6  ± 0.2	1.2  ± 0.2
Asp54 → Asn	1.4  ± 0.2	1.2  ± 0.2
Asn58 → Ala	1.5  ± 0.1	1.1  ± 0.2
Asn77 → Ala	1.3  ± 0.2	1.4  ± 0.2

^*Mutations were generated in an Ile⁷⁶ → Val + Ile⁸⁸ → Val + Ile⁹⁶ → Val mutant background. 

^†Determined in normal import buffer. Errors are SE. 

^‡Determined in low ionic strength import buffer A. Errors are SE. 

^§I is the ionic strength of import buffers.