Figure 5.

Presentation of peptide level solvent accessibility for Aβ(1-40). (a) The peptide level solvent accessibility pattern of Aβ(1-40) in different aggregated states. The deuterium labeling times for monomer (light gray) and LMW oligomers (gray) from fresh sample, HMW oligomers (dark gray) from 4-h aged sample, and fibrils (squared) from 6-day washed sample are 10 s, 1 h, and 24 h, respectively. The horizontal dash dot line corresponds to 100% solvent accessibility at full deuteration. (b) Aβ reporter regions with significant difference in solvent accessibility between two sequential aggregated states. Bars span peptide regions that are significantly different (significance level α is 0.05) for three pairwise comparisons: (LMW-MON) LMW oligomers versus monomer, (HMW-LMW) HMW versus LMW oligomers, and (FIB-HMW) fibrils versus HMW oligomers. No bars are shown for regions without significant differences.