Table 4.
System |
KSV (pH 6.5) |
KSV (pH 7.4) |
||
---|---|---|---|---|
5NS | 16NS | 5NS | 16NS | |
POPC/POPG | 2.07 | 0.36 | 0.82 | 1.29 |
POPC/POPG/DAHP | 2.25 | 1.83 | 4.11 | 1.83 |
POPC/POPG/LPS | 2.86 | 0.36 | 4.60 | 1.68 |
Stern-Volmer constants (KSV) of rBPI21 were obtained as described in Materials and Methods using 5NS and 16NS quenchers at 37°C, pH 6.5 and pH 7.4 Anionic lipid contents in lipid mixtures: 40% molar; LPS or DHAP content in mixtures: 5%.