Figure 1.
Western blot analysis of whole saliva using an anti-human BDNF antibody revealed immunoreactivity for pro- and mBDNF. Forty ug of total salivary proteins from each participant were separated by SDS-PAGE and analyzed through immunoblotting. This procedure revealed bands at 14 and 24 kD, as well as doublet bands at 32 and 34 kD; 2.5 ng of rBDNF were run as a positive control. The relative amount of each form of the protein varied considerably between individuals.