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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Arch Oral Biol. 2009 May 20;54(7):689–695. doi: 10.1016/j.archoralbio.2009.04.005

Figure 3.

Figure 3

Treatment of a pooled saliva sample with plasmin demonstrated total cleavage of salivary proBDNF and lower molecular weight glycosylated forms to mBDNF. Whole saliva was incubated with either PBS (“C”) or plasmin (“E”) for 1 hr at 37°C. The products were separated by SDS-PAGE and analyzed by Western blot. Treatment with the protease resulted in the cleavage of proBDNF, as well as the lower molecular weight proteins, to mBDNF. The relative molecular weight of mBDNF was not affected by plasmin treatment.