Fig. 4.

RegIIIγ expression is triggered by intestinal bacteria. (A) RegIIIγ expression along the cephalocaudal axis of the small intestine. Small intestines from adult germ-free or conventionalized (conv-D) NMRI mice were divided into 16 equal segments (numbered proximal to distal) and RegIIIγ mRNA expression was determined in specific segments using Q-PCR. Results are representative of experiments in two sets of mice. (B) RegIIIγ mRNA increases during the weaning period (P17 to 22) in developing conventionally raised NMRI mice. Assays were performed on pooled mid–small intestinal RNAs (for three mice per time point). (C) RegIIIγ expression is triggered by single Gram-positive or Gram-negative bacterial species in immunodeficient mice. Q-PCR determinations were done on cDNAs from mid–small intestine. Each point represents the average value from three or more different mice. All Q-PCR determinations were performed in triplicate (mean ± SD plotted) and were normalized to 18S ribosomal RNA.