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. Author manuscript; available in PMC: 2009 Jul 28.
Published in final edited form as: Analyst. 2009 Apr 1;134(6):1067–1076. doi: 10.1039/b821166c

Fig. 5.

Fig. 5

Panel (a): 4-cluster spectral image, constructed via HCA in the 1800–1496 cm−1 and 1350–900 cm−1 spectral ranges, of the unstained and paraffinised tissue area shown in Fig. 3(a). Panel (b): 13-cluster spectral image obtained from the same HCA described in panel (a). Panel (c): 13-cluster spectral image, constructed via HCA in the 1350–900 cm−1 spectral range. Panel (d): mean 2nd derivative spectra calculated for the yellow (capsule), white (T-lymphocyte), royal blue (B-lymphocyte), green (histiocyte), dark blue (breast micro-metastasis, cytoplasmic region) and red (breast micro-metastasis, highly nucleated region) regions of panel (b).