Figure 7. Segregation of DV axons in the optic tract (deltaOT) at PND1.
Axons labeled after focal injection into dorsal or ventral retina travel in lateral or medial optic tract, respectively. A. Example of axon label in the delta of the optic tract after a dorsal retinal injection. Red line indicates where quantification of fluorescent label across the optic tract is performed. Medial is left, lateral is right. B. Example of axon label in the delta of the optic tract after a ventral injection. Again medial is left, lateral is right. Note the label is shifted toward the medial side of the tract. C. Profile of the fluorescent label in the delta of the optic tract for (n=8) dorsal injections. D. Profile of the fluorescent label in the delta of the optic tract for (n=8) ventral injections. Black arrows in C and D mark the mean of the center of mass of the fluorescent label, which is much different for the dorsal (C) and ventral (D) injections (P<0.001). Scale bar is 250 μm in A and B.
