Figure 8. Position of dorsal and ventral axons in basal optic tract at PND1.
Animals received paired ventral (A, red) and dorsal (B, green) injections in one eye on the day of birth. Horizontal sections of the optic tract within 200μm of the optic chiasm reveal a spatial segregation of the two labels (A, B, overlay in C). Another example is shown in F, to illustrate the division of the tract into medial and lateral sectors for quantitative comparison. Rostral is to the right in A,B,C, and F. Quantification of the distribution of label for the top quartile of fluorescence shows a differential distribution of dorsal and ventral label in the medial (D) and lateral (E) halves of the tract, with dorsal axons running preferentially in the medial optic tract, and ventral axons in the lateral optic tract. Scale bar is 125 μm in panels A, B, C and F.
