Figure 3. Effect on Matrigel invasion.
A) A549 cells were plated in the upper chamber of a transwell coated with Matrigel with DMSO or AZD0530 at 1 μM. After 48 hours, cells invading through the Matrigel were photographed. B) A549 and Calu-6 cells invading through the Matrigel were fixed, stained with 0.5% methylene blue and counted. Results presented as a percentage of the A549 DMSO control (±SEM of quadruplicate wells). C) A549 cells were incubated with AZD0530 at indicated doses for 24 hours, followed by FAK-immunoprecipitation and Western blotting for pY576-FAK and total FAK.