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A) Basal expression levels of pY705-STAT3 and total STAT3 were determined by Western blotting in A549, Calu-1, Calu-6, H69 and H526 cell lines. B) A549 cells were incubated with 500 nM of AZD0530 for the times indicated, followed by Western blotting for pY705-STAT and STAT3. C) A549 cells were incubated with indicated doses of AZD0530 for 24 hours, followed by Western blotting for pY705-STAT3 and STAT3.
