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A and B) A549 and Calu-6 cells were incubated with AZD0530, PP2 or LY294002 at the concentrations indicated for 24 hours and then not irradiated (0 Gy), or irradiated with doses of 2, 5 or 10 Gy. After 72 hours, MTT was used to assess cell viability. C) A549 cells were incubated with PP2 (10 μM) or AZD0530 (500 nM) for the times indicated, followed by Western blotting for pS473-AKT.
