Skip to main content
. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: J Immunol. 2009 Jan 1;182(1):711–717. doi: 10.4049/jimmunol.182.1.711

Figure 1. Induction of DNA fragmentation in the mouse epidermal cells following PF IgG passive transfer.

Figure 1

(A) TUNEL staining. Neonatal mice were injected (s.c.) with pathogenic PF or normal human IgG. Skin samples were obtained 20 h post IgG injections and subjected to TUNEL staining. Positive TUNEL labeling was revealed in the epidermis from mice (n=6) injected with IgG from PF1 or PF2, but not from control mice (n=3) injected with the normal human IgG. Representative results are shown. v: vesicle. (B) Oligonucleosome releasing assay. DNA oligonucleosomes presented in the cytoplasmic fractions of mouse epidermal sheets obtained from control mice (n=2) and PF mice (n=2) were analyzed with an ELISA-based assay for presence of the histone-DNA complex.