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Detection and basic characterization of proteases in skin mucus extract of rainbow trout at pH 6.8 by 2D-gel zymography. A) Detection of protease activities with TCA fixation after IEF. B) Without TCA fixation after IEF. C) Identification of cysteine proteases by treatment with E-64 without TCA fixation after IEF. D) Identification of cysteine proteases by treatment with CA-074 without TCA fixation after IEF.
