a–h: Azan-Mallory staining of livers from mice treated with CCl4 after gene transduction. Upper panels, AAV5-LacZ-transduced mouse livers (a: 3 weeks, b: 6 weeks, c: 9 weeks, and d: 12 weeks after AAV-5 LacZ transduction). Lower panels, AAV5-HGF-transduced mouse livers (e: 3 weeks, f: 6 weeks, g: 9 weeks, and h: 12 weeks after AAV-5 HGF transduction). Original magnification 40×. (I): Assessment of fibrosis using image analysis techniques, calculating the ratio of connective tissue to the whole area of the liver from mice transduced with AAV5-LacZ or AAV5-HGF. Data are presented as the mean ± SD (5 fields per mouse, n = 5)