Gel images illustrating the Western blot assay of protein abundance. The following genes were tested for their protein abundance: GCK, glucokinase; EGFR, epidermal growth factor-like module containing mucine-like, hormone receptor-like receptor sequence 4 (or Emr4); Cyp450 reducatse, and β-actin. Other abbreviations: SD, standard diet; HCD, high carbohydrate diet. The following values apply for the HCD-to-SD ratio: GCK, 2.00 ± 0.18; Cyp450 reductase, 0.16 ± 0.08; EGFR, 1.23 ± 0.28