Fig. 5.

Human liver myofibroblasts bind FITC-labelled C1-3 scAb (green). Hepatic stellate cells were isolated by pronase and collagenase perfusion from resected human liver and were cultured as outlined [11]. After transdifferentiation and sub-culture, cells were typically more than 95% positive for the classic myofibroblast marker α-smooth muscle actin [1]. The panel shows a typical view of liver myofibroblasts after incubation with FITC-labelled C1-3 (green) in culture as outlined [16], followed by fixation and co-staining for the myofibroblasts marker α-smooth muscle actin (red) and DNA using DAPI (blue)