Fig. 7.

Time course for the effects of C1-3-GT on active caspase 3 levels in human myofibroblasts in vitro. Human myofibroblasts were cultured and treated with C1-3-GT essentially as outlined in Figure 5. At the indicated time points, cells were fixed and stained for α-smooth muscle actin (red), active caspase 3 (green) and DNA using DAPI (blue). The active caspase 3 antibody was purchased from Promega (Southampton, UK). Results are typical of cells isolated from 3 individual patients