Table 1. Molecular masses of subunits and native aggregates of wild-type αB-crystallin and its mutants.
| Protein | Subunit (Da) | Native aggregate (kDa) |
|---|---|---|
| WT |
20131 |
550 |
| Δ1 |
20001 |
400 |
| Δ2 |
19873 |
370 |
| Δ5 |
19634 |
500 |
| Δ7 |
19433 |
460 |
| Δ10 |
19136 |
450 |
| Δ11 |
19008 |
480 |
| Δ12 |
18879 |
n.d. |
| Δ1/K174A |
19942 |
420 |
| Δ1/K174S |
19957 |
330 |
| Δ1/K174E |
20001 |
370 |
| K174A |
20070 |
620 |
| K175A |
20071 |
570 |
| K174/175A |
20013 |
570 |
| K174/175E | 20131 | 630 |
The subunit masses were analyzed by using an LCQ mass spectrometer and native molecular masses determined by analytical gel-permeation FPLC. In the table, n.d. indicates not determined.