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. 2009 Jul 28;15:1429–1444.

Table 1. Molecular masses of subunits and native aggregates of wild-type αB-crystallin and its mutants.

      Protein       Subunit (Da)       Native aggregate (kDa)
      WT
      20131
      550
      Δ1
      20001
      400
      Δ2
      19873
      370
      Δ5
      19634
      500
      Δ7
      19433
      460
      Δ10
      19136
      450
      Δ11
      19008
      480
      Δ12
      18879
      n.d.
      Δ1/K174A
      19942
      420
      Δ1/K174S
      19957
      330
      Δ1/K174E
      20001
      370
      K174A
      20070
      620
      K175A
      20071
      570
      K174/175A
      20013
      570
      K174/175E       20131       630

The subunit masses were analyzed by using an LCQ mass spectrometer and native molecular masses determined by analytical gel-permeation FPLC. In the table, n.d. indicates not determined.