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. 2009 Aug;175(2):519–532. doi: 10.2353/ajpath.2009.090016

Figure 2.

Figure 2

Overexpression of Nrf2-dependent HO-1 inhibits VCAM-1 and ICAM-1 expression in HTSMCs. Cells were pretreated with CoPP IX for 16 hours in the presence or absence of ZnPP IX, and then treated with TNF-α for (A) 24 hours or (B) 5 hours. The protein levels and mRNA expression of VCAM-1 and ICAM-1 were determined. C: Cells were transiently transfected with an ICAM-1- or VCAM-1-luc reporter gene, pretreated with CoPP IX for 16 hours in the presence or absence of ZnPP IX, and then treated with TNF-α for 4 hours. The luciferase activity of ICAM-1 or VCAM-1 was measured. D, E: HTSMCs were pretreated with CoPP IX for 16 hours in the presence or absence of ZnPP IX, and then treated with TNF-α for 24 hours. The (D) THP-1 cells adherence and (E) IL-6 generation were measured. F, G: Cells were pretreated with CoPP IX for 16 hours, transfected with siRNA of HO-1, Nrf2, or scrambled, and then incubated with TNF-α for 24 hours. The (F) levels of VCAM-1 and ICAM-1 expression and (G) IL-6 generation were measured. Data are expressed as mean ± SEM of three independent experiments. Significant differences between the compared groups are indicated: *P < 0.05; #P < 0.01.