Figure 5.

MMPs lie downstream of TGF-β1 in repressing E-cadherin transcription by β-catenin. A: Relative E-cadherin promoter activity (firefly versus Renilla luciferase activities) in NRK52e cells transiently co-transfected with mouse E-cadherin promoter (−201 to +131) Firefly luciferase reporter construct and control Renilla luciferase construct, and treated with rMMP-3 proteolytic domain or TGF-β1 alone or with GM6001 (25 μmol/L) or anti-TGF-β1 Ab 1D11 (30 μg ml⁻¹). B: Relative E-cadherin promoter activity by co-transfection with WT β-catenin or vector control. Data are expressed as mean ± SD (n = 3). P values represent statistical significance assessed by one way analysis of variance followed by post hoc Fisher PLSD test in comparison with control.