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IGFBP-5-induced ECM and α-SMA production is regulated by the MAPK pathway. A: Fibroblasts were infected with Ad-IGFBP-5 (Ad5) or cAd. Activation status of MEK1/2 was analyzed by Western blot. B: Fibroblasts were treated as in A following a 1-hour incubation with 10 μmol/L MEK inhibitor U0126 (Meki) or DMSO as a vehicle control (veh). ECM and α-SMA levels were analyzed by Western blot.
