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. 2009 Aug;175(2):605–615. doi: 10.2353/ajpath.2009.080991

Figure 4.

Figure 4

Nuclear translocation of IGFBP-5 is not regulated by MAPK signaling in primary human lung fibroblasts. A: Fibroblasts were infected with Ad-IGFBP-5 (Ad5), Ad-IGFBP-3 (Ad3), or cAd at an MOI of 50. Cytoplasmic and nuclear fractions were extracted. Expression of IGFBP-3 and -5 was examined by Western blot. β-actin is shown as a loading control for cytoplasmic extracts, and histone H3 for nuclear extracts. B: Fibroblasts were infected with Ad-IGFBP-5 or cAd at an MOI of 50 following pretreatment with MEK inhibitor (MEKi) or DMSO as vehicle control (veh). Cytoplasmic and nuclear fractions were extracted. Expression of IGFBP-5 was examined by Western blot.