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Increased Egr-1 expression in IPF. A: Egr-1 levels in primary fibroblasts from three patients with IPF and three healthy donors were analyzed by Western blot. B: Graphical summary of Egr-1 levels. Intensity of the bands on Western blot was analyzed using ImageJ and the ratio of Egr-1 to GAPDH was calculated. *P < 0.05. C: Egr-1 levels were detected using immunohistochemistry in lung tissues of three patients with IPF and three normal donors. Magnification: ×400; ×800 (Inset).
