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. 2009 Aug;175(2):605–615. doi: 10.2353/ajpath.2009.080991

Figure 8.

Figure 8

IGFBP-5 exerts similar effects in primary mouse and human lung fibroblasts. A: IGFBP-5 co-localizes with Egr-1 and IGFBP-5 induces Egr-1 in non-IGFBP-5-expressing mouse fibroblasts in a paracrine manner. Expression of Egr-1 (red) and IGFBP-5 (green) was examined by immunofluorescence. DAPI was used for nuclear counterstaining. Arrows show paracrine induction of Egr-1 in non-IGFBP-5-expressing cells. Images were taken at ×1200 magnification on a confocal microscope. B: IGFBP-5 and Egr-1 co-localize in the peri- and intranuclear cellular regions. Images were reconstructed with X-Z and Y-Z axis using MetaMorph. Yellow signal denotes co-localization. Images were taken at ×2000 on a confocal microscope. C: IGFBP-5 activates MAPK signaling in wild-type and Egr-1 KO mouse lung fibroblasts. Fibroblasts were stimulated with 500 ng/ml recombinant IGFBP-5. Protein phosphorylation was analyzed by Western blot.