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Co-immunoprecipitation of CD81 and GPC3. The rat liver lysates without heparitinase treatment were incubated with anti-CD81 monoclonal antibody or control IgG, followed by precipitation with agarose A/G plus beads. Precipitates were separated by Western blotting and probed with anti-GPC3 rabbit polyclonal antibody. Arrow indicates 89-kd GPC3 protein.
