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. 2009 Aug;175(2):891–902. doi: 10.2353/ajpath.2009.081115

Figure 9.

Figure 9

Level of nitrite+nitrate, nitrite, and superoxide in arginase deficient mice. Wild-type mice (WT) or partially deficient in arginase (Arg1+/−Arg2−/−) were treated with LPS (4 mg/kg, i.p.) for 16 hours. A: Retina lysates were incubated with nitrate reductase to reduce nitrate to nitrite. Then the level of nitrite was determined by NO analyzer. B: Nitrite level in the retina lysates was determined by NO analyzer. C: Real-time DHE imaging of superoxide formation in retinas from LPS treated WT and Arg1+/−Arg2−/− mice or from control mice. Representative images and statistic of fluorescence intensity are shown. Wild-type mice without LPS treatment were used as control (Con). For (A) and (B) n = 3 mice for control and n = 9 mice for LPS treated mice. For (C), n = 3 mice for each group. #P < 0.05 compared with control. *P < 0.05 compared with WT+LPS.