Figure 3.

The PBAF complex contains both common and distinctive subunits compared with BAF. (a) A silver-stained SDS-gel showing PBAF purified with BAF180 antibody (lane 2). BAF complex was shown as a control (lane 1). Unique components are marked with arrows. The presence of IgG is indicated by an asterisk. Several polypeptides that have been further identified by mass spectrometry analysis were denoted with #. (b and c) Immunoblot analysis of the load, flowthrough (FT), and eluate fractions from a BAF180 antibody column. The doublet bands of BAF180 may represent isoforms from alternatively spliced mRNA. (d) Immunoblot analysis of elution profile of a Superose 6 gel-filtration chromatography to estimate the molecular mass of PBAF.