Figure 6.

Absence of protein in RGS11 mutant mice. A, Top panel, Western blot analysis of retinal lysates (1/15 of a retina) from two RGS11 mutant mice and two wild-type mice using anti-mouse RGS11 serum R4970. Whereas the RGS11 antiserum detected a single band (∼50 kDa) with the expected size of RGS11, no band at that position was detected in the lanes containing retinal lysates from RGS11 mutant mice. Bottom panel, Western blot analysis of the same blot with GAPDH antibody to show that comparable amounts of proteins were loaded. B–G, Loss of immunostaining for RGS11, but not other antigens, in RGS11 mutant mice. The bright staining for RGS11 (red) postsynaptic to ribeye staining (green) in the outer plexiform layer (C) is eliminated in mice homozygous for the targeted disruption of the RGS11 gene (F). In contrast, staining for RGS7 (red, B, E) and staining for Gβ5 (D, G) were unaffected by disruption of the RGS11 gene. Scale bars: B–G, 10 μm.