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. Author manuscript; available in PMC: 2010 Jan 16.
Published in final edited form as: Oncogene. 2009 May 18;28(28):2556–2568. doi: 10.1038/onc.2009.118

Figure 4. Dose and Time dependent expression of Bip and CHOP, ER stress markers and accumulation of ubiquitinated proteins in 15d-PGJ2 treated cells showing cytoplasmic vacuolation.

Figure 4

A. Western blot showing expression of ER stress markers, Bip, CHOP and ubiquitinated proteins upon treatment with indicated doses of 15d-PGJ2 for 9h. B. Time course expression of Bip, CHOP and ubiquitinated proteins with 20µM of 15d-PGJ2 by western blotting at indicated hours. GAPDH is used as a loading control. C. Distribution of Bip, CHOP, VCP and GAPDH in detergent soluble (S) and insoluble low (P1) and high (P2) speed pellet fractions with or without 15d-PGJ2 treatment (20 µM) for 9h. Effect of transcription inhibitor, actinomycin D (20 µg/ml) and translation inhibitor, cycloheximide (25 µM) on cytoplasmic vacuolation induced by15d-PGJ2. D. Phase contrast images E. Cell viability as measured by clonogenic assay. F. Inhibition of accumulation of ubiquitinated proteins in detergent insoluble fractions P1 and P2 by act D and CHX as shown by western blotting with ubiquitin antibodies.