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Schematic experimental setup for the conversion of (+)-valencene (1) by CYP109B1 in a biphasic system. (+)-Valencene (1) is converted to cis-nootkatol (2), trans-nootkatol (3) and (+)-nootkatone (4) by recombinant E. coli expressing CYP109B1, putidaredoxin reductase (PdR) and putidaredoxin (Pdx). The organic phase acts as substrate reservoir and allows the accumulation of the mono-oxygenated conversion products, which prevents them from overoxidation.
