Table 2.

Cytokine elaboration from human monocyte-derived macrophages following exposure to M. genitalium G37^a.

Human MDM
	Viable	UV-Inactivated	PBS
IL-1β	31 ± 6.1*	33 ± 1.4*	0.7 ± 0.04
IL-6	385 ± 13.8*	439 ± 4.0*	3.2 ± 0.1
IL-8	5784 ± 149*	5368 ± 564*	116 ± 7.8
G-CSF	63.1 ± 5.5*	72 ± 2.4*	6.2 ± 0.1
IFN-γ	270 ± 24*	339 ± 3.9*	9 ± 3.6
MCP-1	298 ± 9.3*	318 ± 8.3*	36 ± 3.9
MIP-1α	1056 ± 16*	1068 ± 4.0*	176 ± 10.9
MIP-1β	2514 ± 57*	2403 ± 19*	810 ± 47
RANTES	66 ± 1.5*	74 ± 9.9*	11.4 ± 0.4
TNF-α	7456 ± 334*	8616 ± 697*	20 ± 2.0

^a Human MDM were inoculated with M. genitalium G37 (MOI 10) or an equal volume of the PBS vehicle as a control into triplicate wells. Culture supernatants were collected 6 h PI to quantify secreted cytokines as described in the Methods. Values are expressed as the mean ± SEM pg/mL supernatant. PBS values are presented to indicate basal cytokine elaboration. Data collected following exposure to M. genitalium strain M2300 were similar in pattern and magnitude. ND, not detected. *, p < 0.01 using ANOVA.