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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Gastroenterology. 2009 May 13;137(2):618–628. doi: 10.1053/j.gastro.2009.05.002

Figure 4. Hh pathway activity across the E16.5 pyloric border.

Figure 4

(A, B) In situ hybridization of antrum using Shh and Ihh specific probes. (C-F) X-gal stained antral sections from E16.5 Ptch1LacZ/+, Gli1LacZ/+, and Gli2LacZ/+ antrum. (F) Cross section of the forming pyloric border in a Gli1 LacZ/+ mouse reveals a drastic difference in active Hh signaling between the stomach (S, left) and intestine (I, right); Py = pylorus. Arrowheads = Gli1 positive serosal cells. Bars = 100μm.