Figure 2.

Rlp7p localizes to the nucleolus. Immunofluorescence microscopy was performed with a strain expressing an HA-tagged Rlp7p. Rabbit anti-HA antibodies were used to detect HA-Rlp7p (A) and the mouse mAb A66 was used to detect the nucleolar protein Nop1p (fibrillarin) (B). Nuclear DNA was stained with 4′,6-diamidin-2-phenylindole (C). The images were merged (D); the yellow staining results from overlapping of the green and red signals.